By Marissa Boucher and Kimberlee West
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Extra info for 1500 Poses A Boudoir, Glamour, and Portrait Shot Guide for Photographers and Models
Therefore, co-injection of MCT2 and embigin cRNA is required for robust expression of MCT2 in Xenopus oocytes . 28 Holger M. Becker MCT1, MCT2, MCT4, and embigin have been cloned into the Xenopus oocyte expression vector pGEM-He [1, 27–29]. From these vectors cRNA of MCTs and embigin can be produced by in vitro transcription for heterologous expression in Xenopus oocytes. 2 pH-Sensitive Microelectrodes Since MCTs transport lactate in cotransport with H+ in a 1:1 stoichiometry, pH-sensitive microelectrodes are a very powerful tool to measure activity of MCTs in Xenopus oocytes.
Dev Neurosci 15(3–5):226–232 Dienel GA, Wang RY, Cruz NF (2002) Generalized sensory stimulation of conscious rats increases labeling of oxidative pathways of glucose metabolism when the brain glucoseoxygen uptake ratio rises. J Cereb Blood Flow Metab 22(12):1490–1502 Tildon JT, Stevenson JH, Roeder LM (1987) Serum effects on substrate oxidation by dissociated brain cells: possible sites of action. Brain Res 403(1):127–135 Hawkins RA et al (1985) Cerebral glucose use measured with [14C]glucose labeled in the 1, 2, or 6 position.
Biochem J 304:751–760 4. Gerhart DZ, Enerson BE, Zhdankina OY et al (1997) Expression of monocarboxylate transporter MCT1 by brain endothelium and glia in adult and suckling rats. Am J Physiol 273:207–213 5. Lee Y, Morrison BM, Li Y et al (2012) Oligodendroglia metabolically support axons and contribute to neurodegeneration. Nature 487:443–448 6. Pierre K, Magistretti PJ, Pellerin L (2002) MCT2 is a major neuronal monocarboxylate transporter in the adult mouse brain. J Cereb Blood Flow Metab 22:586–595 7.
1500 Poses A Boudoir, Glamour, and Portrait Shot Guide for Photographers and Models by Marissa Boucher and Kimberlee West
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